Biotechnology Letters, Vol.23, No.7, 555-558, 2001
Leaf developmental stage and tissue location affect the detection of beta-glucuronidase in transgenic tobacco plants
Expression in Nicotiana tabaccum L. plants containing the beta -glucuronidase (GUS) gene under the control of the 35S (CaMV promoter) was affected by tissue type and ontogenic development of the leaves. GUS activity in ontogenetically younger leaves was 1003-1022 nmol 7-hydroxy-4-methylcoumarin (MU) formed mg(-1) (protein) min(-1) and in ontogenetically older leaves was only 140-198 nmol (MU) mg(-1) (protein) min(-1).