Biotechnology Letters, Vol.23, No.11, 887-891, 2001
Production of optically pure L-alanine in batch and continuous stirred tank reactors using immobilized Pseudomonas sp.
A process to obtain optically pure L-alanine has been developed using batch and continuous stirred tank reactors with a new L-aminoacylase-producing bacterium Pseudomonas sp. BA2 immobilized in calcium alginate beads coated with glutaraldehyde. The maximum production of L-alanine in a continuous stirred tank reactor was 11.26 g after 2 days of operation which is higher than that previously reported.