The sugar composition of inulo-oligosaccharides produced from inulin consisted of mainly inulobiose and inulotriose in almost equal amounts with a bacterial endoinulinase either as a soluble enzyme or as intact cells. Inulobiose was the major product with immobilized enzyme or cells. The composition in fructo-oligosaccharides which had similar structure to inulo-oligosaccharides showed no significant difference irrespective of enzyme sources and reaction modes applied.