Biotechnology Letters, Vol.19, No.10, 961-965, 1997
Regulated Toluene Cis-Glycol Production by Recombinant Escherichia-Coli Strains Constructed by PCR Amplification of the Toluene Dioxygenase Genes from Pseudomonas-Putida
The toluene dioxygenase genes from Pseudomonas putida NCIMB 11767 were isolated by PCR amplification from recombinant plasmid, p1/1. The genes were subcloned into pUC18 and pKK223-3 and expressed under the lac and tac promoters, respectively. In both cases, toluene cis-glycol was produced, with higher levels of product formation when the genes were expressed from the tac promoter.