Kinetics of heterogeneous nucleation of n-alcohols (C20OH and C22OH) on vapor-deposited thin films of fatty acid is studied both experimentally and theoretically. A kinetic model describing the three-dimensional nucleation and subsequent growth on active centers is solved numerically. The basic characteristics of nucleation process, such as nucleation rate and the total number of nuclei on unit surface, are determined from the distribution function. Depletion of active centers on template film has been incorporated into our computation. The interfacial energy was chosen to get best coincidence between theoretical and experimental data of the total number of nuclei, which gives 0.0029 J m(-2) for C20OH and 0.00285 J m(-2) for C22OH, respectively.