Synthesis of capsaicin analogs using natural oils as an acyl donor has been achieved by condensation with vanillylamine using Lipase D, Lipase R or Novozym 435 as a catalyst. When olive oil was employed, the major product was olvanil (1, 15.8-20.3%). Safflower oil gave a mixture of two major products, 1 (11.4-12.6%) and linoleoyl vanillylamide (2, 12.5-13.4%). The major product from perilla oil was linolenoyl vanillylamide (3, 7.5-10.0%).