Biotechnology Letters, Vol.21, No.7, 635-639, 1999
Acylation of sucrose with vinyl esters using immobilized hydrolases: demonstration that chemical catalysis may interfere with enzymatic catalysis
The acylation of sucrose with vinyl laurate in dimethylsulfoxide was catalyzed by Celite (28% conversion in 24 h at 40 degrees C, 150 mg catalyst ml(-1)), Eupergit C (11% conversion in 24 h at 60 degrees C, 150 mg catalyst/ml), and even the simple Na2HPO4 (17% conversion in 24 h at 40 degrees C, 20 mg catalyst ml(-1)). These chemical acylations must therefore be taken into account in acylations of hydroxyl-containing compounds with enol esters in polar solvents using immobilized enzymes.