화학공학소재연구정보센터
Biotechnology Letters, Vol.21, No.8, 701-705, 1999
Kinetics of cell growth and secondary metabolism of a high-tanshinone-producing line of the Ti transformed Salvia miltiorrhiza cells in suspension culture
When cultivated in 6,7-V medium in suspension culture, Salvia miltiorrhiza, transformed with Agrobacterium tumefaciens C58, grew rapidly, reaching about 9.7 g l(-1) dry wt after 12 days. The cell line produced tanshinones: 150 mg cryptotanshinone, 20 mg tanshinone I and 50 mg tanshinone IIA/l and phenolic acids: 530 mg rosmarinic acid and 216 mg lithospermic acid B/l. The phenolic acids were intracellular while about 1/3 of the tanshinones were extracellular. This is the first report of simultaneous production of both phenolic acids and tanshinones in a single culture system.