Simultaneous recognition of two chiral centers in alpha,beta-diastereomeric amino acids by an N-carbamoyl-L-alpha-amino acid amidohydrolase from Alcaligenes xylosoxidans

Ogawa J; Ryouno A; Xie SX; Rakesh VM; Indrati R; Miyakawa H; Ueno T; Shimizu S

Biotechnology Letters, Vol.21, No.8, 711-713, 1999

DOI10.1023/A:1005592615103 Export Citation

Simultaneous recognition of two chiral centers in alpha,beta-diastereomeric amino acids by an N-carbamoyl-L-alpha-amino acid amidohydrolase from Alcaligenes xylosoxidans

Ogawa J, Ryouno A, Xie SX, Rakesh VM, Indrati R, Miyakawa H, Ueno T, Shimizu S

Cells of Alcaligenes xylosoxidans containing N-carbamoyl-L-alpha-amino acid amidohydrolase strictly distinguished the configuration of not only the alpha-carbon but also the beta-carbon of N-carbamoyl-beta-methylphenylalanine, and produced threo-L-beta-methylphenylalanine specifically from a mixture of the four stereoisomers.