The steady-state method for measuring the volumetric gas-liquid oxygen transfer coefficient based on the liquid phase k(L)a and liquid-solid oxygen transfer coefficient k(S) using air oxidation of glucose catalyzed by the immobilized glucose oxidase gel beads has been established in a bubble column in which there is an axial distribution of the gel beads along the column height. The authors' previous work showed that the method was successfully applied to an external or internal airlift bubble column since either column has an uniform distribution of the gel beads due to a recirculating liquid flow. The reasonable determinations of the k(L)a and k(S) have been obtained taking into account the observed steady-state axial distributions of both gel beads and dissolved oxygen concentrations as well as an inhibitory effect of the intermediate product, hydrogen peroxide on the oxidation rate. Two methods for determining the oxygen transfer parameters k(L)a and k(S), one being for the overall average oxygen transfer properties and the other for the local properties along the column height, have been proposed based on the authors' previous simplified model for the oxygen transfer with air oxidation of glucose catalyzed by the gel beads immobilizing both glucose oxidase and manganese dioxide particles for an efficient production of calcium gluconate. The former method has provided the average k(L)a and k(S) values in a good agreement with the literature values obtained usually by the physical dynamic method. The latter method has presented the local values of k,a and k,, which are both almost independent of the column height in spite of the axial distribution of the gel beads. This is probably the first report on the determination of k(L)a and k(S) taking into account the observed axial distribution of the reacting particles in the normal bubble column.