Pyronarine (PY) is a novel antimalarial drug. The interaction of hemin with PY was investigated in salt bridge supported bilayer lipid membrane (Sb-BLM) system. Sb-BLM was modified with anionic site lauric acid anions (LA). Then, hemin was also adsorbed and incorporated in Sb-BLM by electrostatic force between hemin and LA. Hemin showed well cyclic voltammetry behavior. The cathodic peak current I,, of hemin decreased in the presence of PY, which illustrated the interaction between hemin and PY. The I,, decreases proportional to the increase of PY concentration from 2.75 x 10(-9) to 3.02 x 10(-5) mol(-1), which is the basis for the determination of PY. The interacting mechanism was studied and the optimum conditions for assay of PY were also investigated.