Polymers containing 8-hydroxyquinoline moiety were prepared. Modifications of the base polymer of glycidyl methacrylate were carried out in order to introduce chloromethyl groups, either by the hydrolysis of the poly(glycidyl methacrylate) and the chloroacetylation of the hydrolyzed polymer by the reaction with chloroacetyl chloride or by aminating the poly(glycidyl methacrylate) either with ethylenediamine or with hexamethylenediamine, followed by reacting the aminated polymers with chloroacetyl chloride. The polymers containing 8-hydroxyquinoline moiety were prepared by reacting the chloromethyl groups containing polymers with potassium salt of 8-hydroxyquinoline. The antimicrobial activity of the polymers obtained was examined against gram-negative bacteria (Escherichia coli) and gram-positive bacteria (Bacillus subtilus) as well as the fungus Trichophyton rubrum. Generally, all three polymers proved effective against the tested microorganisms, but growth inhibitory effects varied from one another.