In vitro techniques were used to evaluate the interactions between Fibroblastic cells and Poly(p-Dioxanone) PPDX and compared its performance with that of other polymeric substrates. In vitro biocompatibility was assessed by studying cell adhesion and cell growth of cells on the polymer films themselves as well as in media enriched with the degradation products of PPDX and Poly(glycolic)/Poly(L-lactide 90:10 copolymer (PGLA-910). Our results show that althought all polymers tested were suitable for initial attachment, PPDX proved to be the most favorable surface for cell growth; as cell density after 48 h of culture was similar to that obtained on tissue culture Polystyrene TCPS (control). No signs of cell damage were detected using scanning electron microscopy (SEM) where after 48 h. of cell seeding on PPDX, fibroblast exhibited a confluent cell multilayer similar to TCPS. In addition, the products of the hydrolytic degradation of PPDX had no citotoxic effect on the adherence and proliferation of fibroblastic cell on TCPS. The hydrolytic degradation starts in the amorphous regions, as the tie-chain segments in these regions degrade into fragments causing the pH decrease in buffer solution and weight loss of degradable polymers. The in vitro evaluation suggests that PPDX may be candidate biomaterial for the construction a cell-polymer matrix.