Site-selective adhesion of hydroxyapatite (HAp) microparticles to NH2-terminated self-assembled monolayers (NH2-SAM, positive surface) and OH-SAM (negative) was investigated in the present paper. HAp microparticles were measured to be negatively charged, and homogeneously formed at 50 degreesC in the simulated body fluid (SBF) with high supersaturation degree (1.5 SBF; the concentrations of Ca2+ and pO(4)(3-) alone are 1.5 times that in 1.0 SBF) buffered carefully at 7.6 - 7.7. The zeta potential of microparticles decreased from -11.5 mV to neutrality owing to the absorption of cations accompanied by the simultaneous growth of microparticles. The micropattern of HAp particulate films was successfully fabricated by the use of site-selective adhesion of HAp microparticles on the positive NH2-SAM owing to the attractive electrostatic interactions. The quantitative analysis using quartz crystal microbalance also confirmed the identical phenomena.