Anaerobic digestion of night soil was carried out in a 25 l digester at 10 degrees C using an adapted inoculum. Biogas production was studied at 20, 25, 30, 35 and 40 days hydraulic retention time. Digesters operated at 25 day hydraulic retention time produced 69.72 l biogas/kg VS/day with a methane content of 73.0%. Microbial counts and enzyme activities are also reported.