Adsorption rates of gibberellic acid (GA3) on S-8 and X-5 resins were measured with the results indicating that the adsorption attained equilibrium for adsorption times longer than 4h. The adsorption isotherms of GA3 on AB-8, X-5, S-8, D4020, D3520, D4006 and NKA-9 were determined at 20 degrees C. S-8 resin had the largest solid/liquid distribution coefficient of 10.5 and was selected as the adsorbent for separation of GA3 from the aqueous solution. The breakthrough curves of GA3 from the aqueous solution on S-8 resin were determined at different flow rates. The dynamic adsorption capacity of GA3 was measured as 4.56 mgg(-1) wet resin at a flow rate of 0.5 cm(3)min(-1). GA3 adsorbed on S-8 resin was eluted with several eluents with 80% (v%) acetone aqueous solution having the best desorption performance. When GA3 was adsorbed by S-8 resin from the fermentation Liquor, the dynamic adsorption capacity of GA3 was increased to 12.02 mgg(-1) wet resin in virtue of the salting-out effect of the ammonium sulfate in the fermentation liquor. The yield of GA3 was above 90% after GA3 was adsorbed by S-g resin from the fermentation liquor at pH 2 and eluted by 80% (v%) acetone aqueous solution. The GA3 concentration was increased seven-fold from the fermentation Liquor after an adsorption and desorption cycle.