Transglycosylations are an important class of enzyme-catalysed reaction that occur in most living organisms and which are finding increasing application for the synthesis of therapeutic compounds. Compared with other bioconversion processes, however, they generally suffer from low product yields. This is due to the fact that in aqueous environments water is able to undergo a nucleophilic attack of the enzyme-substrate complex, increasing the rate of the competing hydrolysis reaction. The equilibrium yield of such reactions is consequently only around 10% (w/w). Here, the potential of applying in-situ product removal (ISPR), with the boronate-containing affinity resin Affi-Gel (R) 601, to the alpha -glucosidase mediated conversion of phenyl alpha -D-glucoside to phenyl alpha -maltoside has been examined. ISPR can increase the product yield from such kinetically-controlled reactions by removing the product from the bulk aqueous phase as soon as it is formed. In this way the competing hydrolysis reaction can be prevented and conversions potentially driven to completion. Initial experiments revealed that the optimum pH of the alpha -glucosidase reaction in water-acetonitrile mixtures was between 5.5 and 6.5, whereas the optimum pH for binding of the product to the Affi-Gel (R) 601 resin was between 8.0 and 8.5. Despite having to compromise on both the optimal conditions for glucosidation and for binding, an increase in product yield of 25% (w/w) was still possible following the implementation of ISPR at pH 8 in an aqueous medium containing 50% (v/v) acetonitrile. Similar results were found with the beta -galactosidase catalysed synthesis of phenyl alpha -galactobiose, indicating the potentially generic nature of the ISPR methodology. While these initial results are promising, they indicate the need for more highly selective resins for carbohydrate adsorption (with higher capacities) if further increases in product yield are to be obtained.