Journal of Bioscience and Bioengineering, Vol.89, No.5, 485-488, 2000
Sequence analysis of a beta-agarase gene (pjaA) from Pseudomonas sp isolated from marine environment
The pjaA gene of Pseudomonas sp. W7 consists of an open reading frame of 1926 bp encoding beta-agarase, a protein of 642 amino acids and a molecular weight of 69,540 Da. The expressed protein of plasmid pEAG3-3, In which 259 amino acid residues from C-terminus of the overexpression plasmid (pEAG3) were eliminated, led to the complete loss of agarolytic activity.