The traditional extraction procedure of safflower was complex and difficult to reproduce. The systematical characteristic data for the extraction and seperation of carthamin are insufficient. In this study, we have investigated the applicable extraction method and the various methods to establish the optimum extraction process of carthamin. The extraction solvents and pH were varied in order to explore the optimum condition of alkaline and acidic solvent on the each extraction processes. The absorbance of the extracted precarthamin and carthamin were also studied to identify the amount of each relative extraction with a UV-visible spectrophotometer. The scientific chromaticity coordinate was analyzed to stabilize and reproduce the natural dyes. It has been found that the optimum condition of alkaline and acidic solvent is pH 11.5 of sodium carbonate and pH 4.0 of 40% acetic acid. The optimum mixed solvent was sodium carbonate and potassium hydroxide of 8:2 in volume ratio and acetic acid and hydrochloric acid of 6:4 in volume ratio. The coordinate data of optimum condition was distributed red region on CIE chromaticity diagram.