A simulated moving bed adsorptive reactor (SMBR) has been developed and tested experimentally to conduct a regioselective enzyme-catalyzed diol esterification in a hexane solvent. The reaction is equilibrium limited, and accumulation of water on the biocatalyst causes a reduction in biocatalytic activity. As a result simultaneous removal of water by adsorption on a catalytically inert ion-exchange resin in Na form has been used to improve conversion. A three zone SMBR system was developed for this purpose integrating reaction, adsorption, and regeneration. The packed beds in the three zones are periodically advanced in a "merry-go round" fashion to simulate countercurrent flow. The SMBR allows continuous operation while reducing desorbent consumption and improving conversion relative to a conventional fixed-bed reactor. A mathematical model was developed to simulate the SMBR system based on independent analyses of adsorption and reaction phenomena. The model takes into account the interplay of these phenomena and provides a useful tool to understand the effects of process variables and for the selection of optimum operating conditions.