화학공학소재연구정보센터
Journal of Chemical Engineering of Japan, Vol.35, No.4, 393-397, 2002
Elucidation of the expression mechanism of the synergistic effect on hydrolysis of crystalline cellulose by cellulases
FIE, FIIIE, FIIE and FIVE were purified from Meicellase CEP-6000 as cellulose components. The former two were tentatively named CMCase-type components and the latter two, MCCase-type components. To confirm the previous finding that the fragmentation of MCC with FIVE played an important role in the expression of the synergistic effect, one of FIE, FIIIE and FIVE was adsorbed to MCC at a low temperature (3 C) for a constant time (24 h or 96 h) before starting the hydrolysis (pre-adsorption). On the basis of the findings obtained, the effective adsorption of FIVE to MCC in the early stage of the reaction appeared to raise the synergistic effect, but the excessive adsorption of FIE or FIIIE to MCC in the early stage of the reaction did to inhibit an effective adsorption of FVIE to MCC and consequently to lower the synergistic effect. It was confirmed that, as proposed previously by Tanaka et al. (2000), the expression of the synergistic effect was closely related with the adsorption of FIVE as a pure MCCase-type component to MCC and the fragmentation of MCC by the MCCase-type component in the early stage of the reaction. However, the influence of the pre-adsorption on the synergistic effect was limited in the early stage of MCC hydrolysis.