화학공학소재연구정보센터
Journal of Chemical Engineering of Japan, Vol.35, No.9, 900-907, 2002
Dispersion of proteins in annular chromatography at high angular velocity
The advantage of annular chromatography is the continuous separation of a feed mixture and the simultaneously regeneration of the medium. Therefore it is necessary to know the period, how long such a system can be operated in a continuous manner. In our case the long-term experiment could be performed without a change in performance for one week. Afterwards the peak has started to shift and the variance has increased. In further studies we have demonstrated that increasing the angular velocity has no influence on the resolution of two model compounds. The resolution and HETP (height equivalent to one theoretical plate) stayed constant keeping the loading factor constant. In annular chromatography there are three different dispersions that contribute to peak broadening, axial, radial, and circumferential. The experiments with the stationary annular column showed, that the circumferential dispersion in annular chromatography can be neglected.