Lipase LIP from Pseudomonas aeruginosa, one of nine commercially available hydrolytic enzymes, catalyzed the enantioselective alcoholysis of racemic 4-(1-acetoxy-2,2,2-trifluoroethyl) phenyl acetate with n-butanol, affording (S)-4-(1-hydroxy-2,2,2-trifluoroethyl) phenol at >99% e.e. (E = >100). Moreover, it also showed high enantioselectivity (E = >100) for the alcoholysis of the racemic o-substituted isomer, 2-(1-acetoxy-2,2,2-trifluoroethyl) phenyl acetate.