Derivative of chitosan was synthesized by reacting with palmitic acid. The contact angles on chitosan-palmitate film were measured using dispersion(D), polar(P) and hydrogen(H) liquids. All nonpolar liquids of alkane homologues easily spread on the surface of chitosan-palmitate film. On the other hand, polar or hydrogen-bonding liquids form a certain angle on the surface of chitosan-palmitate film. The critical surface tensions V_c of chitosan-palmitate film were estimated by the Zisman plot(cos θ versus V_L) and other plots. New functional film for MA packaging have been developed to understand the relationship between surface chemistry and wettability of chitosan-palmitate film.