Growth parameters of Pseudomonas aeruginosa were quantified based on steady-state concentrations, utilization rates of glucose and dissolved oxygen, and microorganism concentration in a chemostat that was operated at 25 degreesC, pH 7.2, and an agitation rate 350 rpm. The results showed that the microbial growth was limited by the concentration of glucose and the concentration of oxygen. A dual-substrate, Tessier growth kinetics for oxygen and glucose, was in good agreement with the experimental data using the following biokinetic parameters: mu(max) = 0.29 h(-1), K-g = 26.9 mg/l, K-o = 1. 18 mg/l, Y-x/g = 0.628 g microorganism/g glucose and, Y-x/o = 0.635 g microorganism/g oxygen. Maintenance factors for glucose and oxygen were: m(g) = 0.0078 g glucose consumed/g microorganism h, and m(o) = 0.014 g oxygen consumed/g microorganism h.