The formation of vesicles in the synthetic bolaamphiphiles SEDA/DEAB mixed systems in the temperature range of (25-80 degreesC) was found by FF-TEM. No phase transition temperature was detected in this temperature range by both DSC and fluorescent probe measurements. In addition, the results of the IR spectra showed that the hydrocarbon chains in the mixed systems are in highly ordered arranging state as the temperature increased to higher temperature. All these results showed the superior thermal stability of the vesicles in the SEDA/DEAB mixed systems.