An anion-exchange-group containing porous membrane in the form of hollow fiber was immobilized BSA(bovine serum albumin) as a chiral selector. An anion-exchange group was appended onto a polymer substrate by radiation-induced graft polymerization and subsequent chemical modifications. Two kind of anion-exchange-group namely, Diethylamine and Triethylamine group, were introduced to a polymer brush by reaction of the epoxy group of grafted onto a porous hollow-fiber membrane with Diethylamine and Triethylamine, respectively. DL-Tryptophan solution was forced to permeate through the BSA multilayered porous membrane at a flow 50 mL/h. as a result, Demonstrates that the interaction between BSA and tryptophan was instantaneous, and that diffusional mass-transfer resistance of L-tryptophan to the BSA ligend was negligible.