HPLC법에 의한 알킬페놀류 분석방법

임성현; 박홍순; 소승호; 한상길; 랑문정; 윤여경; Im Sung Hyun; Bak Hong Soon; Noh Seung Ho; Han Sang Kil; Rang Moon Jeong; Yoon Yeo Kyeong

Journal of the Korean Industrial and Engineering Chemistry, Vol.14, No.3, 371-373, May, 2003

Export Citation

HPLC법에 의한 알킬페놀류 분석방법

Analysis of Alkyl phenols by High-Performance Liquid Chromatography

임성현^†, 박홍순, 소승호, 한상길, 랑문정, 윤여경

(주)LG생활건강 기술연구원 생활과학 연구소, 대전 305-343

Im Sung Hyun^†, Bak Hong Soon, Noh Seung Ho, Han Sang Kil, Rang Moon Jeong, and Yoon Yeo Kyeong

R&D Institute, LG Household & Health Care Ltd., Daejeon 305-343, Korea

E-mail:

초록

본 연구는 알킬 페놀류 8종(4-t-butyl phenol (BP), 4-n-pentyl phenol (PP), 4-n-hexyl phenol (HxP), 4-t-octyl phenol (t-OP), 4-n-heptyl phenol (HpP), b-nonyl phenol (b-NP), 4-n-octyl phenol (OP), 4-n-nonyl phenol (NP))을 액체 크로마토그래피(HPLC) 다이오드 배열 검출기(diode array detector, DAD)를 이용하여 동시분석하였다. 분리관은 역상 C18(250 x 4.6 mm), 이동상은 물과 메탄올을 사용하여 기울기 용리(gradient)하에서 알킬 페놀류의 알킬 길이에 따라 분리하였으며, 알킬 페놀류의 검량선을 도시한 결과 좋은 직선성이 보였다. 상기 분석법으로 국내 유통 중인 주방 세제류 내 알킬 페놀류를 분석한 결과 불검출 되었고, 이 때 검출한계는 주입량(10 μL)대비 1 pg이었다.

4-t-butyl phenol (BP), 4-n-pentyl phenol (PP), 4-n-hexyl phenol (HxP), 4-t-octyl phenol (t-OP), 4-n-heptyl phenol (HpP), b-nonyl phenol (b-NP), 4-n-octyl phenol (OP), 4-n-nonyl phenol (NP) were simultaneously separated by a high performance liquid chromatography (HPLC) with UV diode array detector(230 mm, 260 mm, 280 mm) using an octadecylsilica column (C18, 250 X 4.6 mm) and water/methanol gradient solution. The calibration curves of alkyl phenols showed a good linear relationship, and the recoveries were over 95%. With the detection limit of 1 pg for alkyl phenols with respects to the injected amount, alkyl phenols were not detected in the dishwashing detergents that were available on the market.

Keywords:alkyl phenols;HPLC/DAD

[References]

Carlsen E, Br. Med. J., 305, 609 (1992)
Colborn T, Dumanoski D, Meyers JP, Our Stolen Future, Penguin Books, New York, NY (1996)
EDSTAC(1998). Endocrine Disruptor Screening and Testing Advistory Committee (EDSTAC) Final Report, August (1998)
EPA; Endocrine Disruptor Screening Program, What are Endocrine Disruptor, Office of Science Coordination and Policy, August 16 (2000)
Bette H, Chem. Eng. News, 28(May), 13 (1996)
Holt MS, Mckerrell EH, Perry J, Watkinson RJ, J. Chromatogr., 362, 419 (1986)
Antonio M, Walter G, Anal. Chem., 59, 1709 (1987)
Benomar SH, Clench MR, Allen DW, Anal. Chim. Acta, 445, 255 (2001)
Takeda S, Omura A, Chayama K, J. Chromatogr. A, 979, 425 (2002)
Ibrahim NMA, Wheals BB, J. Chromatogr. A, 731, 171 (1996)

[Referenced By]

[1] Carlsen E, Br. Med. J., 305, 609 (1992)

[2] Colborn T, Dumanoski D, Meyers JP, Our Stolen Future, Penguin Books, New York, NY (1996)

[3] EDSTAC(1998). Endocrine Disruptor Screening and Testing Advistory Committee (EDSTAC) Final Report, August (1998)

[4] EPA; Endocrine Disruptor Screening Program, What are Endocrine Disruptor, Office of Science Coordination and Policy, August 16 (2000)

[5] Bette H, Chem. Eng. News, 28(May), 13 (1996)

[6] Holt MS, Mckerrell EH, Perry J, Watkinson RJ, J. Chromatogr., 362, 419 (1986)

[7] Antonio M, Walter G, Anal. Chem., 59, 1709 (1987)

[8] Benomar SH, Clench MR, Allen DW, Anal. Chim. Acta, 445, 255 (2001)

[9] Takeda S, Omura A, Chayama K, J. Chromatogr. A, 979, 425 (2002)

[10] Ibrahim NMA, Wheals BB, J. Chromatogr. A, 731, 171 (1996)