A contact mode scanning atomic force microscope (AFM) probe was found to allow the adsorbed film on mica of dialkyldimethylammonium bromides (DADBs) prepared from their vesicular suspensions to spread in a position-selective way. Such growth of an adsorbed film was shown to be peculiar to double-chain-type surfactants bearing a cationic moiety including DADB and dipalmitoylphosphatidylcholine, and neither cationic single-chain-type surfactants nor anionic double-chain-type amphiphiles exhibited such growth behavior. This type of film growth was suggested to arise from the breakdown of vesicles on the mica substrate caused by the scanning of the contact mode AFM probe because (1) the film growth depended on the magnitude of the force given by the probe and (2) it was observed with adsorbed films prepared from vesicular suspensions but not with those prepared by the Langmuir-Blodgett method. Moreover, this technique was shown to permit the nanoscale patterning of amphiphilie molecules including phospholipids.