Our understanding of microbial catabolic pathways relevant to bioremediation has been shaped by laboratory studies using isolated pollutant-degrading microorganisms. Recent investigations of natural microbial communities have, however, suggested that catabolic populations in the environment are much more diverse than those previously isolated in the laboratory. In addition, most laboratory strains are now thought to constitute minor populations in the environment, sharing only small contributions to bioremediation processes. Currently, attempts to isolate microorganisms that constitute major populations in the environment have been initiated with the aid of molecular ecological techniques. Such studies will provide information more directly relevant to the catabolic reactions occurring in bioremediation processes and are thus expected to help develop new strategies for advancing bioremediation. This article outlines our studies on phenol-degrading bacteria in activated sludge to illustrate a possible scheme of how genetic and physiological information obtained in the laboratory can be applied to advancing bioremediation processes.