Plants not only provide food to humans and animals, but also provide a large number of nonfood products of industrial and chemical importance. Moreover, they have the ability to purify the air, soil and water on the earth. Various trials to genetically improve the potential of plants are actively in progress. Salt-tolerance would be an especially important ability to bestow upon plants for agricultural and industrial purposes, because high salinity conditions are ubiquitous on earth and represent major barriers to growth. Enhancement of resistance against both hyper-osmotic stress and Na+ toxity is necessary for successful molecular breeding of salt tolerant plants. Introduction of genes for osmolyte bio-synthesis is useful to increase hyperosmotic tolerance of plant cells. It is introduced in this review that genetically engineered ectoine synthesis results in increased hyperosmotic tolerance of tobacco cells. High concentrations of Na+ reduce cellular activity by interfering with vital Na+-sensitive enzymes and by affecting K+ transport. Understanding the regulation of K+ and Na+ homeostasis is thus indispensable for enhancement of plant Na+ tolerance. My research group is investigating the Na+ efflux activity of the yeast Na+-ATPase (Enal) when installed in the plasma membrane of plant cells, and the rice K+-Na+ co-transporters (HKT) that contribute to the regulation of K+ and Na+ uptake in root cells.