Journal of Bioscience and Bioengineering, Vol.95, No.4, 421-424, 2003
Cloning, structural analysis and expression of the gene encoding aspartate aminotransferase from the thermophilic cyanobacterium Phormidium lapideum
The aspartate aminotransferase gene from the thermophilic cyanobacterium Phormidium lapideum was cloned and expressed in Escherichia coli. The ORF of 1167 nucleotides encodes a protein of 388 amino acids having a molecular weight of 42,099. A molecular model of PlAspAT shows structural features similar to those of the Thermus thermophilus AspAT.