The adsorption capacity for Ni2+ on to the surface molecular imprinting adsorbent on Penicillium chysogenum mycelium the surface-imprinted adsorbent) was 40 - 45 mg g(-1) (using 200 mg Ni2+ l(-1)), two times of the mycelium adsorbent. The surface-imprinted adsorbent had good stability at pH 2similar to8. The optimal concentration of EDTA for desorption was 0.1 to 0.5 g l(-1). The surface imprinted adsorbent could be reused 15 times without losing its uptake.