화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Journal of Industrial and Engineering Chemistry, Vol.9, No.5, 494-499, September, 2003
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Biosynthesis of a Novel Microbial Tetracopolyester Containing 4-Hydroxyhexanoate by Rhodococcus ruber
Jae-Hwa Lee, and Eun Yeol Lee1, †
  • Department of Bioscience and Biotechnology, Silla University, Busan 617-736, Korea
  • 1Department of Food Science and Technology, College of Engineering, Kyungsung University, Busan 608-736, Korea
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Novel poly (3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) [P(3HB-co-3HV-co-3HHx)] was synthesized from hexanoate by Rhodococcus rubber. The polyhydroxyalkanoate (PHA) synthase of R. ruber can accept thioesters of 3- and 4-hydroxyalkanoates with carbon atoms from four to six. The PHA synthase can also incorporate 4-hydroxybutyrate (4HB) and 4-hydroxyhexanoate (4HHx) monomer into PHA from structurally related substrates. R. ruber could accumulate an unusual PHA consisting of 3HB, 3HV, 3HHx, and 4HHx if the cells were cultured on a mineral salts medium containing 4HHx as a sole carbon source. The structure of polymer was confirmed by gas chromatography-mass spectrometry (GC-MS).
Keywords:GC-MS;4-hydroxyhexanoate;polyhydroxyalkanoate;Rhodococcus ruber
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