Characterization of Poly(ethylene oxide)-b-Poly(L-lactide) Block Copolymer by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

Jeongmin Hong; Donghyun Cho; Taihyun Chang; Woo Sun Shim; Doo Sung Lee

Macromolecular Research, Vol.11, No.5, 341-346, October, 2003

Jeongmin Hong, Donghyun Cho, Taihyun Chang^†, Woo Sun Shim¹, and Doo Sung Lee¹

Department of Chemistry and Center for Integrated Molecular Systems, Pohang University of Science and Technology, Pohang 790-784, Korea
¹Department of Polymer Science and Engineering, Sungkyunkwan University, Suwon 440-746, Korea

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A poly(ethylene oxide)-b-poly(L-lactide) diblock copolymer (PEO-b-PLLA) is characterized by matrixassisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and a block length distribution map is constructed. Although the MALDI-TOF mass spectrum of PEO-b-PLLA is very complicated, most of the polymer species were identified by isolating the overlapped isotope patterns and by fitting the overlapped peaks to the Schulz-Zimm distribution function. Reconstructed MALDI-TOF MS spectrum was nearly identical to the measured spectrum and this method shows its potential to be developed as an easy and fast analysis method of low molecular weight block copolymers.

Keywords:PEO-b-PLLA;block copolymer;MALDI-TOF MS;isotope;molecular weight distribution

[References]

Jeong B, Bae YH, Lee DS, Kim SW, Nature, 388(6645), 860 (1997)
Tanodekaew S, Pannu R, Heatley F, Attwood D, Booth C, Macromol. Chem. Phys., 198, 927 (1997)
Lee HT, Lee DS, Macromol. Res., 10(6), 359 (2002)
Lee SH, Kim SH, Kim YH, Han YK, Macromol. Res., 10(2), 85 (2002)
Glockner G, Gradient HPLC of Copolymers and Chromatographic Cross-Fractionation, Springer-Verlag, Berlin (1992)
Pasch H, Trathnigg B, HPLC of Polymers, Springer-Verlag, Berlin (1997)
Berek D, Prog. Polym. Sci., 25, 873 (2000)
Murphy R, Schure M, Foley J, Anal. Chem., 70, 4353 (1998)
Jandera P, Holcapek M, Kolarova L, J. Chromatogr. A, 869, 65 (2000)
Park S, Cho D, Ryu J, Kwon K, Lee W, Chang T, Macromolecules, 35(15), 5974 (2002)
Belenkii BG, Gankina ES, Tennikov MB, Vilenchik LZ, J. Chromatogr., 147, 99 (1978)
Belu AM, DeSimone JM, Linton RW, Lange GW, Friedman RM, J. Am. Soc. Mass. Spectrom., 7, 11 (1996)
Jackson AT, Yates HT, Scrivens JH, Green MR, Bateman RH, J. Am. Soc. Mass. Spectrom., 8, 1206 (1997)
Schriemer DC, Whittal RM, Li L, Macromolecules, 30(7), 1955 (1997)
Nielen MWF, Mass. Spec. Rev., 18, 309 (1999)
Shin YC, Choi KY, Jin MY, Hong SK, Cho D, Chang T, Ree M, Korea Polym. J., 9(2), 100 (2001)
Cho DY, Park S, Kwon K, Chang TY, Macromolecules, 34(21), 7570 (2001)
Park S, Cho D, Ryu J, Kwon K, Chang T, Park J, J. Chromatogr. A, 958, 183 (2002)
Montaudo MS, Puglisi C, Samperi F, Montaudo G, Macromolecules, 31(12), 3839 (1998)
Montaudo MS, Montaudo G, Macromolecules, 32(21), 7015 (1999)
Lee H, Lee W, Chang T, Choi S, Lee D, Ji H, Nonidez WK, Mays JW, Macromolecules, 32(12), 4143 (1999)
Lee H, Chang T, Lee DS, Shim MS, Ji H, Nonidez WK, Mays JW, Anal. Chem., 73, 1726 (2001)
Cho D, Park S, Chang T, Ute K, Fukuda I, Kitayama T, Anal. Chem., 74, 1928 (2002)
Cho D, Hong J, Park S, Chang T, J. Chromatogr. A, 986, 199 (2003)
Wilczekvera G, Danis PO, Eisenberg A, Macromolecules, 29(11), 4036 (1996)
Wilczek-Vera G, Yu Y, Waddell K, Danis PO, Eisenberg A, Rapid Commun. Mass Spectrom., 13, 764 (1999)
vanRooij GJ, Duursma MC, deKoster CG, Heeren RMA, Boon JJ, Schuyl PJW, vanderHage ERE, Anal. Chem., 70, 843 (1998)

[Referenced By]

[Related Articles]

[1] Jeong B, Bae YH, Lee DS, Kim SW, Nature, 388(6645), 860 (1997)

[2] Tanodekaew S, Pannu R, Heatley F, Attwood D, Booth C, Macromol. Chem. Phys., 198, 927 (1997)

[3] Lee HT, Lee DS, Macromol. Res., 10(6), 359 (2002)

[4] Lee SH, Kim SH, Kim YH, Han YK, Macromol. Res., 10(2), 85 (2002)

[5] Glockner G, Gradient HPLC of Copolymers and Chromatographic Cross-Fractionation, Springer-Verlag, Berlin (1992)

[6] Pasch H, Trathnigg B, HPLC of Polymers, Springer-Verlag, Berlin (1997)

[7] Berek D, Prog. Polym. Sci., 25, 873 (2000)

[8] Murphy R, Schure M, Foley J, Anal. Chem., 70, 4353 (1998)

[9] Jandera P, Holcapek M, Kolarova L, J. Chromatogr. A, 869, 65 (2000)

[10] Park S, Cho D, Ryu J, Kwon K, Lee W, Chang T, Macromolecules, 35(15), 5974 (2002)

[11] Belenkii BG, Gankina ES, Tennikov MB, Vilenchik LZ, J. Chromatogr., 147, 99 (1978)

[12] Belu AM, DeSimone JM, Linton RW, Lange GW, Friedman RM, J. Am. Soc. Mass. Spectrom., 7, 11 (1996)

[13] Jackson AT, Yates HT, Scrivens JH, Green MR, Bateman RH, J. Am. Soc. Mass. Spectrom., 8, 1206 (1997)

[14] Schriemer DC, Whittal RM, Li L, Macromolecules, 30(7), 1955 (1997)

[15] Nielen MWF, Mass. Spec. Rev., 18, 309 (1999)

[16] Shin YC, Choi KY, Jin MY, Hong SK, Cho D, Chang T, Ree M, Korea Polym. J., 9(2), 100 (2001)

[17] Cho DY, Park S, Kwon K, Chang TY, Macromolecules, 34(21), 7570 (2001)

[18] Park S, Cho D, Ryu J, Kwon K, Chang T, Park J, J. Chromatogr. A, 958, 183 (2002)

[19] Montaudo MS, Puglisi C, Samperi F, Montaudo G, Macromolecules, 31(12), 3839 (1998)

[20] Montaudo MS, Montaudo G, Macromolecules, 32(21), 7015 (1999)

[21] Lee H, Lee W, Chang T, Choi S, Lee D, Ji H, Nonidez WK, Mays JW, Macromolecules, 32(12), 4143 (1999)

[22] Lee H, Chang T, Lee DS, Shim MS, Ji H, Nonidez WK, Mays JW, Anal. Chem., 73, 1726 (2001)

[23] Cho D, Park S, Chang T, Ute K, Fukuda I, Kitayama T, Anal. Chem., 74, 1928 (2002)

[24] Cho D, Hong J, Park S, Chang T, J. Chromatogr. A, 986, 199 (2003)

[25] Wilczekvera G, Danis PO, Eisenberg A, Macromolecules, 29(11), 4036 (1996)

[26] Wilczek-Vera G, Yu Y, Waddell K, Danis PO, Eisenberg A, Rapid Commun. Mass Spectrom., 13, 764 (1999)

[27] vanRooij GJ, Duursma MC, deKoster CG, Heeren RMA, Boon JJ, Schuyl PJW, vanderHage ERE, Anal. Chem., 70, 843 (1998)