저분자량의 황산화 키토산과 황산화 알진산 나트륨의 항응고성

김공수; 이지원; 조석형; Kong Soo Kim; Ji Won Lee; Suk Hyung Cho

Polymer(Korea), Vol.27, No.6, 583-588, November, 2003

Export Citation

저분자량의 황산화 키토산과 황산화 알진산 나트륨의 항응고성

Anticoagulation Activities of Low Molecular Weight Sulfated Chitosan and Sulfated Sodium Alginate

김공수^†, 이지원, 조석형¹

충북대학교 공업화학과, 청주 360-763
¹혜전대학 환경/의료재료 계열, 홍성읍 360-763

Kong Soo Kim^†, Ji Won Lee, and Suk Hyung Cho¹

Department of Industrial and Engineerign Chemistry, Chungbuk National University, CheongJu 360-763, Korea
¹Division of Environment and Media Materials, Hejeon Collige, Hongsung-up 350-800, Korea

E-mail:

초록

저분자 키토산과 저분자 알진산 나트륨을 삼산화황-피리딘 복합체와 황산화 반응시켜 황산화 키토산과 황산화 알진산 나트륨을 합성하였다. 황산화 반응에서 삼산화황-피리딘 복합체/다당류의 무게비율이 1:5일 때 황산화도가 각각 2.75와 2.53으로 가장 큰 값을 나타내었다. 합성한 황산화 키토산 및 황산화 알진산의 혈액에 대한 항응고 효과 및 활성트롬보 플라스틴 측정 시험을 행한 결과 분자량이 8.0 x 10³ Da일 때 항응고 효과가 가장 우수하였고 황산화 키토산과 황산화 알진산 나트륨을 무게비율 1:1로 혼합하였을 때 헤파린에 비하여 91% 정도로 항응고 활성이 가장 좋았다. 활성트롬보 플라스틴 측정 시험에서도 황산화 키토산과 황산화 알진산 나트륨의 무게비율이 1:1일 경우에 항응고 활성이 헤파린에 비하여 84%로 가장 좋았다.

Sulfated chitosan and sulfated sodium alginate were synthesized by sulfating reaction of low molecular chitosan and low molecular sodium alginate with SO₃-pyridine complex. When the weight ratio of SO₃-pyridine complex to polysaccharide was 1:5, the degrees of sulfation were the highest at 2.75 and 2.53 respectively. The anticoagulation effect was the highest when the molecular weight was 8.0 x 10³ Da, and the anticoagulation activity was the highest at 91% of that of heparin when sulfated chitosan and sulfated sodium alginate were mixed at a weight ratio of 1:1. The anticoagulation activity was highest at 84% of that of heparin in the active plastin trombo test (aPTT) when sulfated chitosan and sulfated sodium alginate were mixed at a weight ratio of 1:1.

Keywords:sulfated chitosan;anticoagulation

[References]

Craog MJ, Ann. Rev. Biochem., 49, 765 (1995)
Morowitz P, The Chemistry of Blood Coagulation. Spring-Field, IL, Charles C Thomas (1968)
Richard L, Mueller MD, Heparin Circulation, 89, 432 (1994)
Jack H, Valentiv F, Heparin Circulation, 89, 520 (1994)
Lieberman JR, Geerts WH, J. Bone Joint Surg., 76A, 1239 (1994)
Jaques LB, Science, 206, 528 (1978)
Lorner AA, Heparin Chemistry and Clinical Usage, Academic, NY, p. 1218 (1979)
Kumada T, Abiko Y, Thromb. Res., 24, 285 (1981)
Yun YS, Kim KS, Lee YN, J. Chitin Chitosan, 4, 8 (1999)
Muzzarelli RAA, Lough C, Emanuelli M, Cabohydr. Res., 164, 433 (1987)
Fujiki K, Matsuyama H, Yano T, J. Fish Diseases, 17, 349 (1994)
Flanagan DA, Palenik CJ, Setcos JC, Miller CH, Dental Mater., 14, 399 (1998)
Hirano S, Tanaka M, Hasegawa M, Tobetto K, Nishioka A, Carbohydr. Res., 137, 205 (1985)
Nagasawa K, Uchiyama H, Wajima N, Carbohydr. Res., 158, 183 (1986)
Cushing IB, Davis RV, Kratovil EJ, MacCorquodale DW, J. Am. Chem. Soc., 76, 4590 (1954)
Lee CW, Moon SI, Hong YK, Polym.(Korea), 25(3), 385 (2001)
Takeuchi T, Murata K, Kusakaba I, Nippon Shokuhin Gakkaishi, 41, 505 (1994)
Lee RI, White DD, J. Am. Med. Sci., 145, 495 (1913)

[Referenced By]

[1] Craog MJ, Ann. Rev. Biochem., 49, 765 (1995)

[2] Morowitz P, The Chemistry of Blood Coagulation. Spring-Field, IL, Charles C Thomas (1968)

[3] Richard L, Mueller MD, Heparin Circulation, 89, 432 (1994)

[4] Jack H, Valentiv F, Heparin Circulation, 89, 520 (1994)

[5] Lieberman JR, Geerts WH, J. Bone Joint Surg., 76A, 1239 (1994)

[6] Jaques LB, Science, 206, 528 (1978)

[7] Lorner AA, Heparin Chemistry and Clinical Usage, Academic, NY, p. 1218 (1979)

[8] Kumada T, Abiko Y, Thromb. Res., 24, 285 (1981)

[9] Yun YS, Kim KS, Lee YN, J. Chitin Chitosan, 4, 8 (1999)

[10] Muzzarelli RAA, Lough C, Emanuelli M, Cabohydr. Res., 164, 433 (1987)

[11] Fujiki K, Matsuyama H, Yano T, J. Fish Diseases, 17, 349 (1994)

[12] Flanagan DA, Palenik CJ, Setcos JC, Miller CH, Dental Mater., 14, 399 (1998)

[13] Hirano S, Tanaka M, Hasegawa M, Tobetto K, Nishioka A, Carbohydr. Res., 137, 205 (1985)

[14] Nagasawa K, Uchiyama H, Wajima N, Carbohydr. Res., 158, 183 (1986)

[15] Cushing IB, Davis RV, Kratovil EJ, MacCorquodale DW, J. Am. Chem. Soc., 76, 4590 (1954)

[16] Lee CW, Moon SI, Hong YK, Polym.(Korea), 25(3), 385 (2001)

[17] Takeuchi T, Murata K, Kusakaba I, Nippon Shokuhin Gakkaishi, 41, 505 (1994)

[18] Lee RI, White DD, J. Am. Med. Sci., 145, 495 (1913)