Caffeine Molecular Imprinted Microgel Spheres by Precipitation Polymerization

Dexian Wang; Seung Pyo Hong; Gengliang Yang; Kyung Ho Row

Korean Journal of Chemical Engineering, Vol.20, No.6, 1073-1076, November, 2003

DOI10.1007/BF02706938 Export Citation

Caffeine Molecular Imprinted Microgel Spheres by Precipitation Polymerization

Dexian Wang^{1, 2}, Seung Pyo Hong¹, Gengliang Yang³, and Kyung Ho Row^{1, †}

¹Center for Advanced Bioseparation Technology, Department of Chemical Engineering, Inha University, 253 Yonghyun-Dong, Nam-Ku, Incheon 402-751, Korea
²Department of Chemistry, Hebei University, Baoding 071002, Korea
³Department of Chemistry, Hebei University, Baoding 071002, China

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Imprinted uniform microgel spheres were prepared by precipitation polymerization. Acetonitrile was used as the dilute solvent with MAA as the monomer, EDMA as the crosslinker and caffeine as the print molecule. Comparison of caffeine adsorption on molecular imprinted and blank microgel spheres was made. Langmuir model was used to fit the adsorption data. It was found that the caffeine imprinted microgel spheres show specific binding sites to the target molecules. A binding study of caffeine on imprinted microgel spheres was made by Scatchard analysis; the dissociation constants (K_D) and the maximum binding capacity were K_D=1.84×10^-4 mol/L, Q_max=16.98 μmol/g for high affinity binding site and K_D=1.33×10^-3 mol/L, Q_max=46.84 μmol/g for lower affinity binding site, respectively. This microgel spheres can be useful affinity adsorbents in further applications.

Keywords:Molecular Imprinted Polymers;Microgel Spheres;Caffeine

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[1] Ansell RJ, Kriz D, Mosbach K, Curr. Opin. Biotechnol., 7, 89 (1996)

[2] Bereczki A, Tolokan A, Horvai G, Horvath V, Lanza F, Hall AJ, Sellergren B, J. Chromatogr. A, 930, 31 (2001)

[3] Chen W, Liu F, Zhang X, Li KA, Tong S, Talanta, 55, 29 (2001)

[4] Chen Y, Kele M, Quinones I, Sellergren B, Guichon G, J. Chromatogr. A, 927, 1 (2001)

[5] Haginaka J, Kagawa C, J. Chromatogr. A, 948, 77 (2002)

[6] Hosoya K, Yoshizako K, Shirasu Y, Kimata K, Araki T, Tanaka N, Haginaka J, J. Chromatogr. A, 728, 139 (1996)

[7] Kriz O, Ramstrom O, Mosbach K, Anal. Chem., 69, 345A (1997)

[8] Lang CD, Peng H, Bao XY, Nie LH, Yao SZ, Analyst, 124, 1781 (1999)

[9] Matsui J, Miyoshi Y, Doblhoff-Dier O, Takeuchi T, Anal. Chem., 67, 4404 (1995)

[10] Matsui J, Nicholls IA, Takeuchi T, Anal. Chim. Acta, 365, 89 (1998)

[11] Mayees AG, Mosbach K, Anal. Chem., 68, 3769 (1996)

[12] Owens PK, Karlsson L, Lutz ESM, Andersson LI, Trends Anal. Chem., 18, 146 (1999)

[13] Sajonz P, Kele M, Zhong G, Sellergren B, Guiochon G, J. Chromatogr. A, 810, 1 (1998)

[14] Sellergren B, Shea KJ, J. Chromatogr. A, 690, 29 (1995)

[15] Sherrington DC, Chem. Commun., 2275 (1998)

[16] Spivak D, Shea KJ, J. Org. Chem., 64, 4627 (1999)

[17] Ye L, Cormack PAG, Mosbach K, Anal. Chim. Acta, 435, 187 (2001)

[18] Ye L, Mosbach K, React. Funct. Polym., 48, 149 (2001)

[19] Yoshikawa M, Fujisawa T, Izumi J, Kitao T, Sakamoto S, Anal. Chim. Acta, 365, 59 (1998)

[20] Zhang T, Liu F, Chen W, Wang J, Li K, Anal. Chim. Acta, 450, 53 (2001)

[21] Zheng N, Li YZ, Chang WB, Wang ZM, Li TJ, Anal. Chim. Acta, 452, 277 (2002)

[22] Zhou J, He X, Li Y, Anal. Chim. Acta, 394, 353 (1999)