myo-Inositol hexakisphosphate (InsP(6)) was hydrolyzed by a novel phytase purified from Candida krusei. The chemical structures of the product (InsP(1-5)) were identified by an NMR technique. The data indicate that the products from the. enzyme catalytic reaction in sequence are D/L-InS(1,2,4,5,6)P-5, D/L-Ins(1,2,3,5,6)P-5, D/L-Ins(1,2,5,6)P-4, D/L-InS(1,2,5)P-3, D/L-InS(1,2)P-2 and D/L-Ins(2)P-1.