Biotechnology Letters, Vol.25, No.19, 1609-1612, 2003
Reaction of substituted phenols with thermostable laccase bound to Bacillus subtilis spores
A strain of B. subtilis produced 1.8 times more laccase on sporulation medium than on non-sporulation medium. Spores oxidized mono- and di-methoxyphenols (0.1 mM) at 50degreesC. The half-life of laccase bound to spores was about 2 d and the substrate was repeatedly removed by spores recovered from the reaction mixture.