화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Polymer(Korea), Vol.28, No.2, 194-200, March, 2004
Export Citation
돼지의 소장 점막하 조직을 이용한 스폰지의 제조 및 특성 결정
Preparation and Characterization of Sponge Using Porcine Small Intestinal Submucosa
신혜원, 김선화, 장지욱1, 김문석1, 조선행1, 이해방1, 강길선†
  • 전북대학교 유기신물질공학과
  • 1한국화학연구원 나노생체재료연구팀
Hye Won Shin, Seon Hwa Kim, Ji Wook Jang1, Moon Suk Kim1, Sun Hang Cho1, Hai Bang Lee1, and Gilson Khang†
  • Department of Advanced Organic Materials Engineering, Chonbuk National University, 664-14, Duckjin Dong 1Ga, Duckjin Gu, Jeonju 561-756, Korea
  • 1Nanobiomaterials Lab., Korea Research Institute of Chemical Technology, P. O. Box 107, Yuseong, Daejeon 305-600, Korea
E-mail:
초록
돼지의 소장 점막하 조직은 면역반응이 없는 재료로 널리 사용되고 있다. 본 연구에서는 소장 점막하 조직을 1-ethyl-(3,3-dimethyl aminopropyl) carboimide hydrochloride (EDC)를 이용하여 경화시켰으며 동시에 스폰지 형태로 제조하여 조직공학적 담체와 상처 드레싱재료로서의 응용 가능성을 검토하였다. 다세포 구성분을 제거한 소장 점막하 조직 분말을 3% 아세트산에 용해하여 일정한 형태의 몰드 내에 붓고 동결건조하였다. 이 소장 점막하 조직 스폰지를 여러 농도의 DEC 용액에 침지하여 가교반응을 실시하였다. 이를 전자주사 현미경, 시차 주사열량계, 적외선 분광분석기, 다공도 테스트 및 흡수성 실험을 수행하여 특성을 결정하였다. 소장 점막하 조직 스폰지는 경화제의 농도가 50 mM 이상에서 물리적 특성이 우수한 것으로 판단되었다. 일련의 실험을 통하여 경화된 소장 점막하 조직 스폰지가 조직공학적 담체와 상처치료 드레싱재료로 유용하게 사용될 수 있음을 확인하였다.
Porcine small intestine submucosa (SIS) has been widely used as a biomaterial without immunorejection responses. Crosslinked SIS sponges were characterized for the possibility of the bio-interactive wound dressings and tissue engineered scaffolds. SIS powders were dissolved in 3% acetic acid aqueous solution at 48 hrs followed by pouring into mold and then fabricated by freeze-drying method. SIS sponge was prepared by crosslinked with 1-ethyl-(3,3-dimethyl aminopropyl) carbodiimide hydrochloride (EDC) solution (deionized water : ethanol = 5 : 95) with 1 ~ 100 mM concentration for 24 hrs and lyophilized. SIS sponges were characterized by scanning electron microscopy, differential scanning calorimeter, and Fourier transform infrared spectrometer and were tested their porosity and water absorption ability. It was observed that the concentration of EDC might be exceeded 50 mM to get good physical characteristics. In conclusion, it seems that SIS sponge could be very useful for the applications of wound healing and tissue construction.
Keywords:small intestinal submucosa;sponge;collagen;wound dressing;crosslinking
  1. Song SB, Khang G, Hong SK, Lee I, Kim SW, Lee HB, Biomater. Res., 4, 13 (2000)
  2. Lindberg K, Badylak SF, Burns, 27, 254 (2001) 
  3. Badylak SF, SEmin. Cell Devel. Biol., 13, 277 (2002)
  4. Ledet EH, Carl AL, DiRisio DJ, Tymeson MP, Andersen LB, Sheehan CE, Kallakury B, Slivka M, Serhan H, Spine J., 2, 188 (2002) 
  5. Badylak SF, Kropp B, McPherson TB, Tissue Eng., 4, 379 (1998)
  6. Kang JA, Lee SJ, Khang G, Lee I, Lee HB, Biomater. Res., 6, 107 (2002)
  7. Shin PK, Lee SJ, Lee B, Lee YM, Lee HB, Khang G, Macromol. Chem. Symp., 15, 175 (2002)
  8. Khang G, Rhee JM, Shin P, Kim IY, Lee B, Lee SJ, Lee YM, Lee HB, Lee I, Macromol. Res., 10(3), 158 (2002)
  9. Lee I, Lee SJ, Choi JS, Lee JS, Khang G, Lee YM, Lee HB, Childs Nerv. Syst., in press (2004)
  10. Morgan CJ, Coutts AGP, McFadyen MC, King TP, Kelly D, J. Nutri. Biochem., 7, 339 (1996) 
  11. Badylak SF, Lantz GC, Coffey AC, J. Surg. Res., 47, 74 (1989) 
  12. Lantz GC, Badylak SF, Coffey AC, J. Invest. Surg., 3, 217 (1990)
  13. Badylak SF, Kropp B, McPherson TB, Tissue Eng., 4, 379 (1998)
  14. Sandusky GE, Badylak SF, Horff RJ, Annu. J. Path., 140, 317 (1992)
  15. Lamme EN, deVries HJ, vanVeen H, Gabbiani G, Westerhof W, Middelkoop E, J. Histochem. Cytochem., 44, 1311 (1996)
  16. Hong SP, Park JK, Huh SH, Kim HS, J. Plast. Surg., 25, 547 (1998)
  17. Kropp BP, Eppley BL, Prevel CD, Rippy MK, Harruff RC, Badylak SF, Urology, 46, 396 (1995) 
  18. Prevel D, Eppley BL, Badylak SF, Annu. Plast. Surg., 35, 381 (1995)
  19. Rosen M, Ponsky J, Petras R, Fanning A, Brody F, Duperier F, Surgery, 132, 480 (2002) 
  20. Han SK, Choi SJ, "Mechanism of Wound Healing," in Advance in Wound Care, H.J. Yoo, J.H. Kim, M.H. Park, K.K. Lee, J.H. Lee, Y.S. Yoo, S.K. Han, G. Yushimiz, Editors, Korea Medical Book Publisher Co., Seoul, p. 43 (2002)
  21. Yoo JJ, Lee I, Tissue Engineering: Concepts and Application, Korea Medical Book Publisher Co., Seoul (1998)
  22. Chang Y, Tsai CC, Liang HC, Sung HW, Biomaterials, 23, 2447 (2002) 
  23. Charulatha V, Rajaram A, Biomaterials, 24, 759 (2003) 
  24. Pieper JS, Hafmans T, Veerkamp JH, vanKuppevelt TH, Biomaterials, 21, 581 (2000) 
  25. Park SN, Park JC, Kim HO, Song MJ, Suh H, Biomaterials, 23, 1205 (2002) 
  26. Park SN, Lee HJ, Lee KH, Suh H, Biomaterials, 24, 1631 (2003) 
  27. Forato LA, Filho RB, Colnago LA, Anal. Biochem., 15, 136 (1998)