Measurement of red cell deformability and whole blood viscosity using laser-diffraction slit rheometer

Sehyun Shin; Yunhee Ku; Myung-Su Park; Jang-Soo Suh

Korea-Australia Rheology Journal, Vol.16, No.2, 85-90, June, 2004

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Measurement of red cell deformability and whole blood viscosity using laser-diffraction slit rheometer

Sehyun Shin^†, Yunhee Ku, Myung-Su Park, and Jang-Soo Suh¹

School of Mechanical Engineering, Korea
¹Dept. of Pathological Physiology, Kyungpook National University, Daegu 702-701, Korea

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The present study investigated the deformability of red blood cells (RBC) and its effect on whole blood viscosity using a laser-diffraction slit-rheometer (LDSR). The LDSR has been recently developed with significant advances in laser-diffractometry design, operation and data analysis. While shear stress levels in a slit flow are continuously decreasing, both the deformation of red blood cells and the shear stress were simultaneously measured. Additionally, the viscosity of whole blood was measured using the LDSR. The present study found that the whole blood viscosity is strongly dependent on the RBC deformability. The less deformable the RBCs are, the higher the blood viscosity is.

Keywords:slit;deformability;RBC;diffraction;viscosity;stress

[References]

Attali JR, Vaensi P, Diabetes Metab, 16, 1 (1990)
Bareford D, Stone PCW, Calwell NM, Meiselman HJ, Stuart J, Clin. Hemorheol., 5, 311 (1985)
Baskurt OK, Meiselman HJ, Seminars Thrombosis Hemostasis, 29, 435 (2003)
Bessis M, Mohandas N, Blood Cells, 1, 307 (1975)
Bessis M, Mohandas N, Deo C, Blood Cells, 6, 315 (1980)
Chang GS, Koo JS, Song KW, Korea-Aust. Rheol. J., 15(2), 55 (2003)
Chien S, Science, 168, 977 (1970)
Chien S, Principles and Techniques for Assessing Erythrocyte Deformability, in Red Cell Rheology, ed. M. Bessis, S.B. Shohet & N. Mohandas, 71 Springer-Verlag, Berlin (1978)
Chien S, Annu. Rev. Physiol., 49, 177 (1987)
Dobbe JGG, Hardeman MR, Streekstra GJ, Strackee J, Ince C, Grimbergen CA, Blood Cells, Molecules, Diseases, 28, 373 (2002)
Hanss M, Biorheology, 20, 199 (1983)
Hardemann MR, Goedhart PT, Clin. Hemorheol., 12, 381 (1992)
Hardeman MR, Goedhart PT, Dobbe JGG, Lettinga KP, Clin. Hemorheol., 14, 605 (1994)
Holman RC, Herron CA, Sinnock P, Am. J. Public Health, 73, 1169 (1983)
Lowe G, Clinical Blood Rheology, CRC Press, Boca Raton, FL (1988)
Macosko CW, Rheology: Principles, Measurements, and Applications, VCH, New York (1993)
Puniyani RR, Ajmani R, Kale PA, J. Biomed. Eng., 13, 441 (1992)
Shin S, Keum DY, Ku YH, KSME Int. J., 16, 1719 (2002)
Shin S, Ku YH, Park MS, Moon SY, Jang JH, Suh JS, Rev. Sci. Instr., 75, 559 (2004)
Shin S, Lee SW, Ku YH, KSME Int. J., 18, 1036 (2004)
Skovborg F, Nielsen AV, Schlichtkrull J, Ditzel J, Lancet, 1, 129 (1966)
Usami S, Chien S, Bertles JF, J. Lab. Clin. Med., 86, 274 (1975)
Wang X, Zhao H, Zhuang FY, Stoltz JF, Clin. Hemorheol. Microcirc., 21, 291 (1999)

[Referenced By]

[1] Attali JR, Vaensi P, Diabetes Metab, 16, 1 (1990)

[2] Bareford D, Stone PCW, Calwell NM, Meiselman HJ, Stuart J, Clin. Hemorheol., 5, 311 (1985)

[3] Baskurt OK, Meiselman HJ, Seminars Thrombosis Hemostasis, 29, 435 (2003)

[4] Bessis M, Mohandas N, Blood Cells, 1, 307 (1975)

[5] Bessis M, Mohandas N, Deo C, Blood Cells, 6, 315 (1980)

[6] Chang GS, Koo JS, Song KW, Korea-Aust. Rheol. J., 15(2), 55 (2003)

[7] Chien S, Science, 168, 977 (1970)

[8] Chien S, Principles and Techniques for Assessing Erythrocyte Deformability, in Red Cell Rheology, ed. M. Bessis, S.B. Shohet & N. Mohandas, 71 Springer-Verlag, Berlin (1978)

[9] Chien S, Annu. Rev. Physiol., 49, 177 (1987)

[10] Dobbe JGG, Hardeman MR, Streekstra GJ, Strackee J, Ince C, Grimbergen CA, Blood Cells, Molecules, Diseases, 28, 373 (2002)

[11] Hanss M, Biorheology, 20, 199 (1983)

[12] Hardemann MR, Goedhart PT, Clin. Hemorheol., 12, 381 (1992)

[13] Hardeman MR, Goedhart PT, Dobbe JGG, Lettinga KP, Clin. Hemorheol., 14, 605 (1994)

[14] Holman RC, Herron CA, Sinnock P, Am. J. Public Health, 73, 1169 (1983)

[15] Lowe G, Clinical Blood Rheology, CRC Press, Boca Raton, FL (1988)

[16] Macosko CW, Rheology: Principles, Measurements, and Applications, VCH, New York (1993)

[17] Puniyani RR, Ajmani R, Kale PA, J. Biomed. Eng., 13, 441 (1992)

[18] Shin S, Keum DY, Ku YH, KSME Int. J., 16, 1719 (2002)

[19] Shin S, Ku YH, Park MS, Moon SY, Jang JH, Suh JS, Rev. Sci. Instr., 75, 559 (2004)

[20] Shin S, Lee SW, Ku YH, KSME Int. J., 18, 1036 (2004)

[21] Skovborg F, Nielsen AV, Schlichtkrull J, Ditzel J, Lancet, 1, 129 (1966)

[22] Usami S, Chien S, Bertles JF, J. Lab. Clin. Med., 86, 274 (1975)

[23] Wang X, Zhao H, Zhuang FY, Stoltz JF, Clin. Hemorheol. Microcirc., 21, 291 (1999)