Atomic force microscopy (AFM) was used to study the mechanical properties of photo-cross-linked, temperature-responsive hydrogel layers in water. Photo-cross-linkable linear polymers based on N-isopropylacrylamide and 2-(dimethylmaleimido)-N-ethyl-acrylamide were spin-coated to produce uniform thin films of cross-linked responsive hydrogels. These films were imaged using AFM, and force-distance curves were used to measure the temperature-dependent elastic modulus. The volume phase transition of the hydrogel layers is constrained by the presence of a fixed substrate, and the length scale of these effects is related to the modulus. These materials were also studied with surface plasmon resonance and optical waveguide spectroscopy to determine the polymer volume fraction as a function of the temperature. The modulus varies as a function of the polymer volume fraction and is in good agreement with previous measurements on bulk hydrogels. The effect of the cross-linking density and degree of ionization on the modulus was investigated, and a comparison of these results to rubber elasticity theory for a swollen network was used to study the hydrogel morphology. The concentration of elastically active network chains is lower than expected but increases as the network collapses at temperatures above the volume phase transition temperature.