Genome sequencing projects provide an inventory of molecular components for a wide variety of organisms. Metabolic databases integrate these functional descriptions of individual modules into a higher-level characterization of cellular metabolism. This article reviews efforts related to the development of metabolic databases and discusses how such systems have aided the delineation of genome properties. We illustrate the design features of metabolic databases and discuss the challenges facing metabolic as well as databases of other functional types. (C) 2003 Wiley Periodicals, Inc.