Quercetin was anchored to controlled-pore silica glass (CPSG). The chemical bonding of quercetin based on CPSG (QCPSG) was characterized by elemental analysis, infrared reflectance analysis, ultraviolet spectroscopy, and solid state C-13-nuclear magnetic resonance (NMR), in comparison with the monomer phase prepared from quercetin and aminopropyltfihydroxysilane. Controlled-pore silica glass, used as support for quercetin or aminopropyl moiety, showed obvious stability against Si dissolution at pH 8 in comparison with silica gel. Also, the involvement of the amine group in the bonding to quercetin was observed to relieve its local basisity, enabling low capacity fading (10%) after 40 loading/elution cycles. The QCPSG also was used for the separation and preconcentration of Mn(II), Co(II), Ni(II), Cu(II), and Zn(II) prior to their determination by inductively coupled plasma-mass spectrometry (ICP-MS). The optimum pH range for the separation of these metal ions is 7.5-8.5 at 30 min stirring time, giving an efficiency of 98.1%, 94.4%, 95.9%, 90.7%, and 87.9%, respectively, in the presence of sodium acetate. The sorption capacity of QCPSG for these metal ions is in the range of 0.24-0.46 mmol g(-1) indicating a 1:2 quercetin/metal chelation for all metal ions except for Mn(II), as a 1:1 ratio is suggested. The QCPSG was used for the separation and preconcentration of the investigated metal ions in some water samples, using ICP-MS for determination (relative standard deviation, RSD 1.74-6.10%). The method also was applied for the determination of these metal ions in granite ores and certified samples, and the results are in good agreement with the reported values, which indicates that the method is accurate.