Preparation of Electrospun Oxidized Cellulose Mats and Their in vitro Degradation Behavior

Myung Seob Khil; Hak Yong Kim; Young Sic Kang; Ho Ju Bang; Douk Rae Lee; Jae Kyun Doo

Macromolecular Research, Vol.13, No.1, 62-67, February, 2005

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Preparation of Electrospun Oxidized Cellulose Mats and Their in vitro Degradation Behavior

Myung Seob Khil, Hak Yong Kim^†, Young Sic Kang, Ho Ju Bang, Douk Rae Lee , and Jae Kyun Doo¹

Department of Textile Engineering, Chonbuk National University, 664-14 Dukjin, Dukjin, Chonju, Chonbuk 561-756, Korea
¹Department of General Gynecology and Obstetrics med. School, Chonbuk National University, 664-14 Dukjin, Dukjin, Chonju, Chonbuk 561-756, Korea

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This paper investigated the effect of biodegradation behavior on the oxidation of cellulose nanofiber mats. The cellulose mats were produced through electrospinning. The diameter of an electrospun fiber varied from 90 to 240 nm depending on the electrospinning parameters, such as the solution concentration, needle diameter, and rotation speed of a grounded collector. Oxidized cellulose (OC) mats containing different carboxyl contents were prepared using NO2 as an oxidant. The total carboxyl content of the cellulose nanofiber mats obtained after oxidation for 20 h was 20.6%. The corresponding carboxyl content was important from a commercial point of view because OC containing 16-24% carboxyl content are used widely in the medical field as a form of powder or knitted fabric. Degradation tests of the OC mats were performed at 37oC in phosphate-buffered saline (pH 7.4). Microscopy techniques were introduced to study the morphological properties and the degradation behavior of the OC mats. Morphological changes of the mats were visualized using optical microscopy. Within 4 days of exposure to PBS, the weight loss of the OC mats was >90%.

Keywords:electrospun oxidized cellulose mats;electrospinning;carboxyl content;swelling;degradation behavior

[References]

Reneker DH, Chun I, Nanotechnology, 7, 216 (1996)
Deitzel JM, Kosik W, McKnight SH, Tan NCB, DeSimone JM, Crette S, Polymer, 43(3), 1025 (2002)
Buchko CJ, Chen LC, Shen Y, Martin DC, Polymer, 40(26), 7397 (1999)
Fertala A, Han WB, Ko FK, J. Biomed. Mater. Res., 57, 48 (2001)
Huang L, McMillan RA, Apkarian RP, Pourdeyhimi B, Conticello VP, Chaikof EL, Macromolecules, 33(8), 2989 (2000)
Stitzel JD, Pawlowski K, Wnek GE, Simpson DG, Bowlin GL, J. Biomater. Appl., 15, 1 (2001)
Boland ED, Wnek GE, Simpson DG, Pawlowski KJ, Bowlin GL, J. Macromol. Sci., 38, 1231 (2001)
Khil MS, Cha DI, Kim HY, Kim IS, Bhattarai N, J. Biomed. Mater. Res., 67, 675 (2003)
Zussman E, Yarin AL, Weihs D, Exp. Fluids, 33, 315 (2002)
Formhals A, GB Pat. 364780 (1929)
Huang ZM, Zhang YZ, Kotaki M, Ramakrishna S, Compos. Sci. Technol., 63, 2223 (2003)
Gibson PW, Schreuder-Gibson HL, Rivin D, AIChE J., 45(1), 190 (1999)
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Athreya SA, Martin DC, Sens. Actuators A-Phys., 72, 203 (1999)
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Gross U, Muller-Mai C, Voigt C, Fourth World Biomaterials Congress, April, Berlin, Germany, p. 192. (1992)
Chauveaux D, Barbie C, Barthe X, Baquey C, Poustis J, Clin. Mater., 5, 251 (1990)
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Franz G, Adv. Polym. Sci., 76, 1 (1986)
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Banker GS, Kumar V, U. S. Pat. 5,405,953 (1995)
Wiseman DM, Saferstein L, Wolf S, U. S. Pat. 6,500,777 B1 (2002)
Galgut PN, Biomaterials, 11, 561 (1990)
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Kumar V, Yang T, Carbohydr. Polym., 48, 403 (2002)

[Referenced By]

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[1] Reneker DH, Chun I, Nanotechnology, 7, 216 (1996)

[2] Deitzel JM, Kosik W, McKnight SH, Tan NCB, DeSimone JM, Crette S, Polymer, 43(3), 1025 (2002)

[3] Buchko CJ, Chen LC, Shen Y, Martin DC, Polymer, 40(26), 7397 (1999)

[4] Fertala A, Han WB, Ko FK, J. Biomed. Mater. Res., 57, 48 (2001)

[5] Huang L, McMillan RA, Apkarian RP, Pourdeyhimi B, Conticello VP, Chaikof EL, Macromolecules, 33(8), 2989 (2000)

[6] Stitzel JD, Pawlowski K, Wnek GE, Simpson DG, Bowlin GL, J. Biomater. Appl., 15, 1 (2001)

[7] Boland ED, Wnek GE, Simpson DG, Pawlowski KJ, Bowlin GL, J. Macromol. Sci., 38, 1231 (2001)

[8] Khil MS, Cha DI, Kim HY, Kim IS, Bhattarai N, J. Biomed. Mater. Res., 67, 675 (2003)

[9] Zussman E, Yarin AL, Weihs D, Exp. Fluids, 33, 315 (2002)

[10] Formhals A, GB Pat. 364780 (1929)

[11] Huang ZM, Zhang YZ, Kotaki M, Ramakrishna S, Compos. Sci. Technol., 63, 2223 (2003)

[12] Gibson PW, Schreuder-Gibson HL, Rivin D, AIChE J., 45(1), 190 (1999)

[13] Gary L, Bowlin KJ, Pawlowski ED, in Tissue Engineering and Biodegradable Equivalents: Scientific and Clinical Applications, K. U. Lewandrowski, L. W. Donald, J. T. Debra, D. G. Joseph, J. Y. Michael, and E. A. David, Eds., Marcel Dekker, New York, pp 165-78 (2002)

[14] Athreya SA, Martin DC, Sens. Actuators A-Phys., 72, 203 (1999)

[15] Buchko CJ, Slattery MJ, Kozloff KM, Martin DC, J. Mater. Res., 15, 231 (2000)

[16] Buchko CJ, Kozloff KM, Martin DC, Biomaterials, 22, 1289 (2001)

[17] Pommier JC, Poustis J, Baquey C, Chauveaux D, Fr. Pat. 8610331 (1986)

[18] Pommier JC, Poustis J, Baquey C, Chauveaux D, Eur. Pat. 0256906 A1 (1987)

[19] Pommier JC, Poustis J, Baquey C, Chauveaux D, U. S. Pat. 4904258 (1990)

[20] Granja PL, Barbosa MA, Pouysegu L, DeJeso B, Baquey C, in Frontiers in Biomedical Polymers Applications 2, R. Ottenbrite, Ed., Technomic Press, Lancaster, PA, USA, pp 195-225 (1999)

[21] Martson M, Viljanto J, Hurme T, Saukko P, Eur. Surg. Res., 30, 426 (1998)

[22] Gross U, Muller-Mai C, Voigt C, Fourth World Biomaterials Congress, April, Berlin, Germany, p. 192. (1992)

[23] Chauveaux D, Barbie C, Barthe X, Baquey C, Poustis J, Clin. Mater., 5, 251 (1990)

[24] Ikada Y, in Cellulose: Structural and Functional Aspects, J. F. Kennedy, G. O. Phillips, and P. A. Williams, Eds., Ellis Horwood, Chichester, UK, 1989, pp 447-455 (1989)

[25] Miyamoto T, Takahashi S, Ito H, Inagaki H, Noishiki Y, J. Biomed. Mater. Res., 23, 125 (1989)

[26] Franz G, Adv. Polym. Sci., 76, 1 (1986)

[27] Philipp B, Bock W, Schierbaum F, J. Polym. Sci. Polym. Symp., 66, 83 (1979)

[28] Banker GS, Kumar V, U. S. Pat. 5,405,953 (1995)

[29] Wiseman DM, Saferstein L, Wolf S, U. S. Pat. 6,500,777 B1 (2002)

[30] Galgut PN, Biomaterials, 11, 561 (1990)

[31] Roder T, Morgenstern B, Polymer, 40(14), 4143 (1999)

[32] Franklin B, Lowell S, U. S. Pat. 5,180,398 (1993)

[33] Kumar V, Yang T, Carbohydr. Polym., 48, 403 (2002)