Biofilm processes are commonly used for nitrification. Operationally, a whitish heterotrophic biofilm layer tends to develop onto nitrifying biofilm when it has been exposed to organic carbon-containing wastewater for a prolonged period. The development of a heterotrophic biofilm layer could lead to deterioration in nitrification activities and biofilm morphology. The recovery characteristics, in terms of biofilm morphology and nitrification activities, of deteriorated biofilm particles (i.e. nitrifying biofilm particles covered with heterotrophic outgrowth) were investigated by transferring the deteriorated biofilm particles (from an Ultra-Compact Biofilm Reactor (UCBR) which was part of a packed bed-UCBR system used for treating organic carbon and ammonia-containing wastewater) to a UCBR fed solely with ammonia wastewater (referred to as 'Recovery-UCBR'). At a hydraulic retention time (HRT) of 8.7 h and a sand-carrier concentration of 4.0% (v/v), density of the outer heterotrophic biofilm layer reduced progressively which led to subsequent detachment of this layer from the nitrifying biofilm particles. As a result, morphology of the nitrifying biofilm improved gradually in the Recovery-UCBR. A stable nitrification rate of up to 1.74 kg NH4+-N/m(3)d was obtained in the Recovery-UCBR. (C) 2004 Elsevier Ltd. All rights reserved.