화학공학소재연구정보센터
Biotechnology Letters, Vol.26, No.1, 27-30, 2004
Promotion of the growth of Crocus sativus cells and the production of crocin by rare earth elements
La3+ and Ce3+, either singly or a mixture, promoted crocin production of Crocus sativus callus but Nd3+ had little effect and all metal ions were toxic above 100 muM. La3+ (60 muM) promoted growth of callus significantly but increased crocin only slightly. Ce3+ (40 muM) significantly promoted crocin production but had little effect on cell growth. La3+ (60 muM) and Ce3+ (20 muM) together gave the highest dry weight biomass (20.4 g l(-1)), crocin content (4.4 mg g(-1)) and crocin production (90 mg l(-1)) which were, respectively, 1.7-fold, 4.2-fold and 7.1-fold of those without additions. Nd3+ (40 muM) only slightly promoted cell growth and crocin production.