The review considers some problems of process development in the biotechnology and presents their solutions on examples, which were developed in cooperation with industrial partners. These processes include the production of restriktion endonuclease EcoRI by recombinant Escherichia coli, Penicillin V by Penicillium chrysogenum, Xylanase by Aspergillus awamori, Cephalosporin C by Acremonium chrysogenum, Erythritol by Moniliella tomentosa var pollinis, and alkaline serine protease by Bacillus licheniformis. Herewith special attention is called to the practical aspects of the product development.