In this note, a generalized Bezoutian matrix with respect to a polynomial sequence of interpolatory type is introduced. The operator representation relative to a pair of dual bases and the generalized Barnett-type factorization formula are derived. An intertwining relation and a Bezoutian reduction to a block diagonal form by congruence via a generalized Vandermonde matrix are presented. Fujiwara-Hermite and Routh-Hurwitz criteria in terms of this generalized Bezout matrix are obtained.