Colloid-polyrner (CP) mixtures extend between two limiting cases, the colloid limit with the polymer coil size small compared to the colloid radius R,01 and the protein limit with the colloidal particles much smaller in size than the radius of gyration of the polymer chains R-g. In the present work, model systems are developed for the protein limit. The colloid-solvent pairs are optimized in terms of their isorefractivity in order to facilitate the characterization of large polystyrene chains in suspensions of small colloids. The degree of isorefractivity of colloidal particles was successfully evaluated in terms of a reduced scattering intensity. Two polystyrene samples with radii of gyration of R-g = 96 nm and R = 78 nm. respectively, are used. The radii of the colloidal particles are close to R-col = 12 nm, leading to size ratios of R-g/R-col = 8 and R-g/R-col = 6.5. Four colloid solvent systems were found to be suitable for polymer characterization by light scattering, one based on silica particles and three systems with acrylate particles. The present investigation is focused on the three acrylate systems: poly(methyl methacrylate) in ethyl benzoate (ETB) at 7degreesC, poly(ethyl rilethacrylate) in toluene at 7degreesC and poly(ethyl methacrylate) in ETB at 40degreesC. Characterization of PS chains is for the first time performed in colloid concentrations up to 2.5% by weight. In all cases, the size and shape of the polymer chains remain unchanged. A slight mismatch of the colloid scattering or a limited colloid Solubility prevented investigation of PS chains at higher colloid concentration. (C) 2004 Elsevier Inc. All rights reserved.